Analysis of the 3’ untranslated regions of α-tubulin and S-crystallin mRNA and the identification of CPEB in dark- and light-adapted octopus retinas
نویسندگان
چکیده
PURPOSE We previously reported the differential expression and translation of mRNA and protein in dark- and light-adapted octopus retinas, which may result from cytoplasmic polyadenylation element (CPE)-dependent mRNA masking and unmasking. Here we investigate the presence of CPEs in alpha-tubulin and S-crystallin mRNA and report the identification of cytoplasmic polyadenylation element binding protein (CPEB) in light- and dark-adapted octopus retinas. METHODS 3'-RACE and sequencing were used to isolate and analyze the 3'-UTRs of alpha-tubulin and S-crystallin mRNA. Total retinal protein isolated from light- and dark-adapted octopus retinas was subjected to western blot analysis followed by CPEB antibody detection, PEP-171 inhibition of CPEB, and dephosphorylation of CPEB. RESULTS The following CPE-like sequence was detected in the 3'-UTR of isolated long S-crystallin mRNA variants: UUUAACA. No CPE or CPE-like sequences were detected in the 3'-UTRs of alpha-tubulin mRNA or of the short S-crystallin mRNA variants. Western blot analysis detected CPEB as two putative bands migrating between 60-80 kDa, while a third band migrated below 30 kDa in dark- and light-adapted retinas. CONCLUSIONS The detection of CPEB and the identification of the putative CPE-like sequences in the S-crystallin 3'-UTR suggest that CPEB may be involved in the activation of masked S-crystallin mRNA, but not in the regulation of alpha-tubulin mRNA, resulting in increased S-crystallin protein synthesis in dark-adapted octopus retinas.
منابع مشابه
Morphology of retinal photoreceptor layer in continuous light-exposed and dark-adapted male cats
The morphology of retinal photoreceptor layer was studied in continuous light-exposed and dark-adapteddomestic male cats (Felis catus). The eyes of 12 healthy adult cats (4 in continuous light-exposed group, 4 in continuous dark-adapted group, and 4 in control group) were routinely fixed and studied by electron microscope. Results showed that the general structure of photoreceptor layer in this...
متن کاملIdentification of adapted genotypes in sesame lines based on multi-trait selection
Identification of genotypes adapted to a particular region is of special interest to breeders and plant geneticists. In order to identify adapted genotypes in sesame lines based on multi-trait selection, an experiment was conducted in a completely randomized block design with four replications at Moghan Agricultural Research Station of Ardabil Agricultural and Natural Resources Research and Edu...
متن کاملCPEB mediated ZO-1 mRNA Localization is Required for Epithelial Tight Junction Assembly and Cell Polarity
CPEB is a translational regulatory sequence-specific RNA-binding protein that controls germ cell development. Here we show that CPEB heterozygous female mice are fertile but contain disorganized mammary epithelial cells, in which zonal occludens-1 and claudin-3, apical tight-junction proteins, are mislocalized. CPEB depletion from mammary epithelial cells disrupts zonal occludens-1 apical local...
متن کاملتأثیر برهمکنش نور و دما بر جوانهزنی اسفندک (L. Zygophyllum fabago)
Syrian bean caper (Zygophyllum fabago L.) grows in dry and uncultivable wastelands. Because of high tolerance to adverse conditions, an experiment was designed and fulfilled to investigate the effect of two environmental factors, light and temperature, on Syrian bean caper seed germination. Experiment was performed in a completely randomized design with two levels of light (light and dark) and ...
متن کاملI-16: Tubulin Reversible Acetylation – Driving The Moves and The Moves Behind The Drive
Background Asthenozoospermia accounts for almost 50% of the cases of male infertility. Our study investigating phosphoproteins differentially expressed in asthenozoosperm has identified the phosphoproteins relevant to sperm motility and the signature molecules likely to be altered in asthenozoospermia. The 66 phosphoproteins differentially expressed included four alpha tubulin isoforms which we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecular Vision
دوره 14 شماره
صفحات -
تاریخ انتشار 2008